Section 3 of 9
Y-STR genotyping
Julyana Ribeiro, Zehra Köksal, Adriana Castillo, Adriana Ibarra, Beatriz Martinez, Humberto Ossa, Masinda Nguidi, Juan David Granda, Verónica Gomes, Pedro Rodrigues, Maria Inês Machado, Vania Pereira, and Leonor Gusmão · about 1 minutes
All samples were genotyped for 23 Y-STRs using the PowerPlex Y23 kit (PPY23), following the manufacturer’s recommendations (Promega Corporation, Madison, WI). Amplified fragments were separated and detected on a 3500 Genetic Analyzer and genotyped using the GeneMapper™ Software v4.1 (Applied Biosystems).

Fig. 1: Map of Colombia showing sampling locations and the number of samples collected by natural region and Department