Work overview

Section 02 of 03

Material and methods

Establishment of the Stenothemus volaticus group (Coleoptera, Cantharidae), with description of two new species from Xizang, China

Ruobing Hua, Shujuan Ge, Haoyu Liu, Xingke Yang, and Yuxia Yang · 2026

Contents

Section 02 of 03

  1. 01Introduction
  2. 02Material and methods
  3. 03Taxonomy
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Work overview

Section 2 of 3

Material and methods

Ruobing Hua, Shujuan Ge, Haoyu Liu, Xingke Yang, and Yuxia Yang · about 1 minutes

The studied specimens are deposited in the following collections:

IZAS Institute of Zoology, Chinese Academy of Sciences, Beijing, China;

NHMB Naturhistorisches Museum Basel, Basel, Switzerland;

NMPC National Museum, Prague, Czech Republic.

The genitalia of both sexes, along with the female abdominal sternites VIII, were dissected and cleared in 10% NaOH solution. Male genitalia were subsequently immersed in a warm 30% NaOH solution for an appropriate duration (generally 5 to 15 minutes, depending on their size and condition), after which they were cleaned. Female internal genitalia were stained with haematoxylin. Habitus photos were taken using a Canon EOS 80D digital camera, while images of the genitalia were captured with a Leica M205A stereomicroscope. All images were processed using Helicon Focus 7 and edited in Adobe Photoshop CS5. Body length was measured from the anterior margin of the clypeus to the elytral apex, and body width was assessed across the humeral part of the elytra.

For type specimens of the previously known species, complete label data were provided. Square brackets “[]” were used to indicate remarks or comments, [p] denotes printed data, and [h] handwritten data. Quotation marks were used to separate data from different labels, and a backslash “/” to separate data from different lines on the same label. For additional specimens, quotation marks were used when the original labels were written in English, while labels originally in Chinese were transliterated into English.